pucG [2017-8-11 16:0:3]

(S)-ureidoglycine-glyoxylate aminotransferase

locus: BSU32520

pI: 5.42

mw: 45.58 kDa

protein length: 416 aa

gene length: 1248 bp

function: purine utilization

product: (S)-ureidoglycine-glyoxylate aminotransferase

essential: no

synonyms: yurG

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]

Gene

Coordinates: 3,341,166 → 3,342,416

The protein

Catalyzed reaction/ biological activity

• transamination between an unstable intermediate ((S)-ureidoglycine) and the end product of purine catabolism (glyoxylate) to yield oxalurate and glycine [Pubmed|20852637]

Protein family

• class-V pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

Structure

• [PDB|3ISL] [Pubmed|20852637]

Expression and Regulation

Operons

• genes: [gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]-[gene|4BC4B3E0AB4779D1DD5EDF591361FC1094AF661F|pucG]
  description: [Pubmed|12029039]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]

  regulation

  • induced in the presence of purine nucleotides (inducer: allantoin) ([protein|search|PucR]) [Pubmed|12029039]

Biological materials

Mutant

• MGNA-A942 (yurG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/942 NBRP B. subtilis, Japan]

References

• 11344136,20852637,12029039